產(chǎn)品詳情
簡單介紹：
重組大鼠心肌營養(yǎng)素-1蛋白與其它公司提供的重組蛋白不同，rRtCT-1蛋白產(chǎn)品為采用CFS的無細胞麥胚蛋白合成系統(tǒng)表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組大鼠心肌營養(yǎng)素-1蛋白（全長序列）產(chǎn)品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組大鼠心肌營養(yǎng)素-1蛋白
| Species | Rat |
| Accession | Q63086 |
| GeneID | 29201 |
| Source | Escherichia coli. |
| Molecular Weight | 重組大鼠心肌營養(yǎng)素-1蛋白Approximately 21.4 kDa, a single non-glycosylated polypeptide chain containing 203 amino acids. |
| Quantity | 2μg/10μg/1000μg |
| AA Sequence | MSQREGSLED HQTDSSFSFL PHLEAKIRQT HNLARLLTKY ADQLLEEYVQ QQGEPFGLPG FSPPRLPLAG LSGPAPSHAG LPVSERLRQD AAALSALPAL LDAVRRRQAE LNPRAPRLLR SLEDAARQVR ALGAAVETVL AALGAAARGP VPEPVATSAL FTSNSAAGVF SAKVLGLHVC GLYGEWVSRT EGDLGQLVPG GVA |
| Purity | > 95 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重組大鼠心肌營養(yǎng)素-1蛋白Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 106 IU/mg. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 30 % Acetonitrile, 0.1 % TFA. |
| Endotoxin | Less than 1 EU/μg of rRtCT-1 as determined by LAL method. |
| Reconstitution | 重組大鼠心肌營養(yǎng)素-1蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 4mM HCl to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組大鼠心肌營養(yǎng)素-1蛋白 | |
| SDS-PAGE |
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| Reference |
1. Pennica D, Wood WI, Chien KR. 1996. Cytokine Growth Factor Rev. 7:81-91. 2. Robledo O, Fourcin M, Chevalier S, et al. 1997. J Biol Chem. 272:4855-63. 3. Gerhard DS, Wagner L, Feingold EA, et al. 2004. Genome Res. 14:2121-7. |
| Background | Cardiotrophin-1 (CT-1) is a member of the cytokine family which also includes IL-6, IL-11, leukemia inhibitory factor (LIF), oncostatin M (OSM), and ciliary neurotrophic factor (CNTF). CT-1 is a pleiotropic cytokine which is expressed in various tissues including the ***** heart, skeletal muscle, ovary, colon, prostate and fetal lung. Studies showed CT-1 which induces cardiac myocyte hypertrophy in vitro can bind to and activate the ILST/gp130 receptor. Rat CT-1 encodes a 203 amino acid (a.a.) residue protein that lacks a hydrophobic signal peptide and it shares 94 % a.a. and 79 % a.a. sequence identity with human and murine CT-1. |
